Part:BBa_K844017
Spider Silk Generator - 10x "F" Silk Construct with His Tag
Protein generator part for Spider Silk. Protein is a 10x fusion of spider silk "F" subunits (1x BBa_K844007 followed by 9x BBa_K844003), which were codon optimized to use a minimal set of tRNA codons (1 codon per amino acid). Contains a lactose/IPTG inducible promoter, RBS, and a 10x-His tag (BBa_K844000) for purification of protein.
IMPORTANT NOTE: This part uses Assembly Standard #23 (Silver Fusion) scar sites, which the sequence data below and on composite parts using this part will not reflect this scar sequence (it shows Assembly Standard #10 scars).
Related Parts
BBa_K844000 - A 10x-His tag with double stop codons (TAATAA) which was vital to the creation of the gel and blot shown above.
BBa_K844002 - The non-optimized native spider silk subunit that K8440016 is a codon optimized and improved concatemer of.
BBa_K844016 - Another silk generator, this part uses the "B" subunit and has only 4x repeat units.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |